a FastaFile multiple sequence alignment nucleotide sequence collection for scan the SNP variation sites. [as FastaFile]
ref.index
the index number of the reference sequence in the input multiple sequence alignment collection, which is used as the reference for make the SNP calling. [as string]
pureMode
only accept the pure SNP variations? if this parameter is TRUE, then the SNP sites that contains mixed variation information will be ignored. [as boolean]
monomorphic
include the monomorphic sites in the SNP scanning result?. [as boolean]
vcf.output.filename
a by-ref parameter for get the file path of the generated VCF format SNP variations output file. [as String&]