| {rockhopper} | R# Documentation |
require(GCModeller);
#' A system for analyzing bacterial RNA-seq data
imports "rockhopper" from "rnaseq";
Rockhopper is a comprehensive and user-friendly system for computational analysis of bacterial RNA-seq data. As input, Rockhopper takes RNA sequencing reads output by high-throughput sequencing technology (FASTQ, QSEQ, FASTA, SAM, or BAM files). Rockhopper supports the following tasks:
Reference based transcript assembly (When one Or more reference genomes are available)
Aligning reads To genomes
Assembling transcripts
Identifying transcript boundaries And novel transcripts such As small RNAs
De novo transcript assembly (When reference genomes are unavailable)
Normalizing data from different experiments
Quantifying transcript abundance
Testing For differential gene expression
Characterizing operon structures
If you make use Of Rockhopper In your work, please cite the following:
A computational system For identifying operons based On RNA-seq data. Brian Tjaden. Methods, 176:62-70, 2020.
De novo assembly Of bacterial transcriptomes from RNA-seq data. Brian Tjaden. Genome Biology, 16:1, 2015.
Computational analysis Of bacterial RNA-seq data. Ryan McClure, Divya Balasubramanian, Yan Sun, Maksym Bobrovskyy, Paul Sumby, Caroline A. Genco, Carin K. Vanderpool, And Brian Tjaden. Nucleic Acids Research, 41(14):e140, 2013.