| FastQ {SMRUCC.genomics.SequenceModel.FQ} | .NET clr documentation |
FASTQ format is a text-based format for storing both a biological sequence (usually nucleotide sequence) and its corresponding quality scores. Both the sequence letter and quality score are each encoded with a single ASCII character for brevity. It was originally developed at the Wellcome Trust Sanger Institute to bundle a FASTA sequence and its quality data, but has recently become the de facto standard for storing the output of high-throughput sequencing instruments such as the Illumina Genome Analyzer.
There is no standard file extension for a FASTQ file, but .fq and .fastq, are commonly used. (http://en.wikipedia.org/wiki/FASTQ_format)
A FASTQ file normally uses four lines per sequence.
Line 1 begins with a '@' character and is followed by a sequence identifier and an optional description (like a FASTA title line).
Line 2 is the raw sequence letters.
Line 3 begins with a '+' character and is optionally followed by the same sequence identifier (and any description) again.
Line 4 encodes the quality values for the sequence in Line 2, and must contain the same number of symbols as letters in the sequence.
header
seuqnece
+quality
一条FastQ序列文件通常使用4行代表一条序列数据：
第一行： 起始于@字符，后面跟随着序列的标识符，以及一段可选的摘要描述信息
第二行： 原始的序列
第三行： 起始于+符号，与第一行的作用类似
第四行： 编码了第二行的序列数据的质量高低，长度与第二行相同
# namespace SMRUCC.genomics.SequenceModel.FQ
export class FastQ extends ISequenceModel {
Headers: string;
IsProtSource: boolean;
Length: integer;
# @M:SMRUCC.genomics.SequenceModel.FQ.FastQ.GetQualityOrder(System.Char) for each char in this string.
Quality: string;
# 第一行的摘要描述信息
SEQ_ID: string;
# the sequence description information text, but usually be string +.
SEQ_Info: string;
SequenceData: string;
}